BISC320-hb-exam1-fall2005

BISC320-hb-exam1-fall2005 - \ «(R/‘9‘Kl .. %ast Name:...

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Unformatted text preview: \ «(R/‘9‘Kl .. %ast Name: EIWV LaLLdagLand time): ‘ fluid/l ll" Multiple choice questions are worth " The structure of an alpha-helix may be: ,fl @amphipathic , / \ bi held together by disulfi e bonds V, ' / "M c) contain a proline every ii‘iamino acidf \/ d) all of the above / \ ./" ' 2. When two atoms differing in electronegativity are joined in a covalent bond, then: a) the electrons are shared equally between the atoms _b) the bond is nonpolar "' ‘ @ the atom with the greater electronegativity’ attracts the bonded electrons more (1) a double bond is formed ” /’ \\ ‘ 5 L e/ 3. (4 pts.) Name two reasons sodium dodecyl sulfate (SDS) is used in denaturing gel electrophoresis of proteins, Give one of these answers in terms of the mass to charge ratio of the proteins. 5V4) Poo/k, W (Yoko/1 , q pug omvf Chan/Q. 5“) g PM a ' e 7' w at {w yum? yum- w I A, (an km W\ KW 7M 5 H t JI WW /I (0419M rW—C (M) .WM W 31mm“ W1 06"») 1 TM (“3W1 [946+ /§‘,ral or WOW! " W .0le {2/ t Wei-W5 - 4. (3 pts. Why do many DNA binding proteins bind palindromic sequences? pV'OXCOK’ .«wtvjytf, murva _,fi ‘ 5. (3pts.) Name three amino acids that may be phosphorylated. , I l WW .1 / “Wm” CH?) ' 6. (4 pts.) How did Avery, McCleod, and McCarty define the “transforming principle” first identified by Griffiths? bu) Val/“V VNUlizbli (Alli/i “AAA VL'VTV'VLWP‘,‘ “WW 3% ((4 Vivi/Vane SWWLS ' loo IfW’NOQ‘l mt MM} x.’< First Name: “(a l‘fl‘l/ - Page Total: \| C8 ,iultiple choice questions are worth 3 points each. Choose the BEST answer for each. 1/ 7. Which of the following dsDNA sequences (only one strand shown) appears to contain a consensus— sequence for a dimeric, sequence—specific DNA binding protein? 21) Ag QA TQgQA ;; 6 T ('3 as signs "a as rage-«swam Which of the following single-stranded DNA molecules will anneal (hybridize) most tightly with this ingle-stranded DNA molecule: 5’-GGATGTGACCTGACAGTAA-3’? a) 5’—AATGACAGTCCAGTGTA8é—§"C AC Tacit“ u“7T GATT b) 5 ’ -GGATGTGACCTGACAGTAA-3’ ‘ 5’-CCTACACTGGACTGTCATT—3’ 5 ’ -TTACTGTCAGGTCACATCC-3 ’ 9. Which of the following regions of a protein is most likely to be buried in its interior (single letter abbreviations for the amino a ids are given in the parentheses)? Lil‘s—G1y-Ala—gly-Agp-Asp—Thr—Gln-Pro (KGAGDDTQP) Ser-Gly—Gln— is-Cys-Ala-Gly-Gln-Val (SGQHCAGNV) @ Ala-Phe—Ala—Ile—Phe—Gly-LeyCLeu—T ‘(AFAIFGLLW) d) Met~Tyr—Thr—Ala-Cys-Glu-Asp—Kgn—gs (MYTACEDNH) 10. (6 pts.) Briefly describe three cellular functions of ATP 1 W I , _ 4‘ {_ _ t v Pltmfliova‘lamfl » (1mm ("4°an ‘4“ firm W *3 5 LL LV-‘Afi MEMO“) /(»‘vmafitm 04> mwt 4.1a knit ‘ .7 {Lflz‘hb‘fl V'j do“ (Tiling q Frigzsflgl,}{ (:QI'WID1d1MJ- WW? W 11. (6 pts.) Draw the tripeptide: Asp-Cys-Lys (from N to C terminus) at QH=7. Some R groups (shown uncharggd)%chopse_from are provided: ' gall alanisfimectly 69V! Hz-OH BT‘ \ gr ’ -CH2-CH2-CiiZ-CH2-NH,/‘ H A 0 (t4; d x‘ O ‘ \ u t / ' .v~ ) . \ H — Midway/cw 45 a x I \ l ' M1 '\ c _ . » r ' 0 a (fog; n H are / ,I \ r COD ‘ 53:1 \ z; N H? 12. (6 pts.) Define primary, secondary, and tertiary levels pr ein s ructure and explain the main chemical bond(s) (or interaction) that stabilizes each level 0 ructure. , . . , ‘ . ‘ -{(r ? “yawn” leywt amMUN‘LC’W WWW“ ‘0') WW” W .x _, . g : impair Ww/C ‘ , , &« Max awash/W5 {Nu/WW 40$ (A, @(tDVyW/C WY W * 23-9 rmww 1mm Let r 1 Ma? «wt up an Jim/heave) (Wyn-towns 2 1 E4 {Zlflllf First Name: V Page Total: iviultiple choice questions are worth 3 points each. Choose the BEST answer for each. / ‘ 13. The active site of an enzyme may contain: a) an acidic amino acid for catalysis b) a basic amino acid for catalysis c) an acidic and basic amino acid for catalysis gall of the above , 14. (8 pts.) In the alpha-helix and beta-schecezt,n , Omit/1 «w» ywww H wen a) which chemical groups form regulai‘ ydrogen bonds with each other? Be specific and show example in a drawing. {that t t ‘3' r’W I‘LVH“V 2 7'6 ’r "M"; " ""’ V , V V ‘ -' /' ' ‘ L“ on Piaf; . [amp/wag) AA A W (W (fitxtfl (C) Haw/La (we fit J4 a? 4 \\ [40 *4? m — c amw — «with, waz 52) Luciltr Mathews?) b) how many alpha-carbons separate the amino acids'that form the H-bonds in the alpha-helix and beta-sheet? H V _ {5M Jg‘zgw “even! 4 logic; (Jean?! Ll/L 'T/(A-i (Wild/O c) use words to explain how the R groups are oriented with respect to the alpha-helix and beta- ‘. A g ,7) ta , 1 sheet. ‘2 q (“1 VS £0101"; C“. u + 5?, am 4‘» snob) A. ' {bu (U93 «cop'th gift/€61 oz 15. (6 pts.) You have isolated three strains of haploid yeast that are ura- (unable to grow on medium lacking uracil), and you name the strains: "X", "y", and "2". After mating each mutant strain with each of the other mutant strains (in the opposite mating types, of course), you observe the following results in the resulting diploid cells: mating growth of diploid on medium lackinguracil strain x with strain y + strain x with strain 2 + strain y with strain z + a) What term describes the result of each mating with {exegt to growth in the absence of uracil? —' -:( \ , - , - , , . f - - . we . .. .x/ é{g(’é€v’€bt\ erflp‘tv’k ~ W1 vwnlflhm vow/V: 16% lAa‘VeM L, ’ 9‘ v . l, . a. C (ix/0Q“ w aw calmer) M M» lch “4 “WWW H W ‘1 ” ' I r _, N w» Mch . ., . a b) What do the results tell you about the identity of the mutation in each strain? AR ‘xwvx u“ 7 .7—-.‘-— _ . A _ _ K Jarth M» ’. 5L Cttkk'mfli’ V‘VM ,k angry (ft/UL} I, L J i X \7/ 1‘ ’ ‘tu 0m ,‘(H W\ L101}? (Lung of amt/u Km ( ’ iv til/Vii 1“ 91» in" [rt/VAL‘UM i 3 \ U/ First Name: l/ Page Total: Multiple choice questions are worth 3 points each. Choose the BEST answer for each. 16. Topoisomerases are: a) single—stranded DNA binding proteins b sequence-specific DNA binding proteins that bind the major groove c enzymes that relax DNA supercoils by stabilizing non—covalent bonds \d) none of the above - )gFor any reaction at equilibrium (Keq): a) catalyst no longer accelerates either the forward or reverse reaction b) the concentration of products equals the concentration of reactants c) AG for the reaction equals zero d) all of the statements are true 18. Which of the following statements regarding double stranded DNA structure is not always true? a) The molar concentration of A+G = C+T 139 The molar concentration of A=T and G=C (9; The molar concentration of A+T = G+C (1) None, all the statements are always true. 19. Which of the following DNA sequences is most likely to be found in the genome of an organism with 69% GC content? [9/ AA’I'I‘AATT b) GGCCGGCC ; c) GGGCCC d) b and'c are equally likely 20. (8 pts.) Draw the structure of each base found in DNA, properly hydrogen bonded with its partner base. Make sure to name each base (full name). Show H-bonds as dotted lines. Show which atom is attached to deoxyribose in DNA by drawing a bond to “R”. 194% “t “r > H i) , «Q? \‘ 6“"? U r/l’\ Poaqavk , W‘s-M // a {garb Oi \ a / N N w P“ @ "WW M") i A 'L. \ v u \k‘ ‘3 « ‘ l~ W First Name: C((PMYV‘W Page Total: 6 Al. (8pts.) Mendel began his experiments with strains of pea plants that bred true (inbred) for one of seven different characteristics. a) what term describes the genotype of the each of his starting straimwfibrgard—tfiaeirgme? ,H WOLWU-éj’jct’s b) how many different types of gametes did each of these starting strains produce (remember that plants are sexual organisms) V] ' ' ’V “I I " ‘ x ‘wg.L‘!>\ t q , 4" me +2394 7411\134 /@ c) beginning with an inbred strain that produced white flowers and round seeds and a second strain 2 not" that produced purple flowers and wrinkled seeds, what phenotypes are observed in the F1 plants and in what proportions? (round seeds and purple flowersare dominant) N N (L r w w ?_‘2_ , , f - o was s; >ng mead Wade 3 , . w a . PW PM. i ‘ wt ‘ M “\AW 4 (h \( w 9‘ v «v p w W4; “Wm/S 43 Ha n7_v’ Mk 4-032? - . s"? :3 *4 (mpg) 9+ “mamas WMK (' 1 myth/S W \yV‘ mm (id 9Q clS X ' d) after allowing the F1 plants to self-pollinate, what phenotypes are observed in the F2 generation and in what proportions? +0 ‘0‘ i a w GOE7- V‘QHVUmgLM3 ‘ K“ W’n’oi r at) (@6495; _ 9D ‘4 [M Le i (“wag 22. (8 pts.) The fouowmg four peptides are separated by 2D gel electrophoresis Label each spot on the/M. mu 4’ gel (A, B, C, or D) to indicate the position of the corresponding peptide (same peptides in single letter 94-11 1% abbreviations are in parentheses). +0 .+( p Peptide A: Ala-Ala Lys—Lys-Val-Va1—Arg-G1u-Pro—Trp-Trp (AAKKVVREPWW) Peptide Trp-Asp—Glu-Asp—Met—Cys—IIe—Ser—Tyr (WDEDMCISY) Peptide C: Met-Phe-A1a—Val-Leu-Leu-G1u-Arg-Trp—Gly—Phe (MFAVLLERWGF) Peptidejj: Met—Phe—Tyr—Ala—Lys—Va1—Lys—Val-Arg—Glu-Trp-Pro«Trp—Ile (MFYAKVKVREWPWI) pH __.__————~_——>10 Direction of migration in second dimension ...
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BISC320-hb-exam1-fall2005 - \ «(R/‘9‘Kl .. %ast Name:...

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