BISCO-320-MT1-Fall2006-key

BISCO-320-MT1-Fall2006-key - Bifit 32H Midterm 1 Answer...

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Unformatted text preview: Bifit' 32H Midterm 1 Answer Key (lilil ptst September 2U, mm; Page 'rnmi: l. ( l0 pts) Please make a simple diagram showing what subsequently happens to two "old” DNA strands (in a double helix) in a parent cell that then divides to become two daughter cells. «id in one daughter and new—old in the other daughter '1: "SQ/‘3“; Ll't.tv [1 “Mil .txigu intuit (flying) '4) '2) [1"5 @ (10 pts) Once attached to a DNA strand. what direction do (a) DNA polymerases and ) RNA polymerases travel on the DNA strand that is being copied? (c) What direction do ribosomes travel on mRNA strands? (Use 5' and 3‘ ends in all three of your answers.) Lam t i *l VJ] ;_ ‘ v ti 3. ( 10 pts) (:1) Using straight lines (parallel to each other) as strands in a diagram. show the 5' and 3‘ ends of double stranded DNA. Designate a transcribed DNA strand and show. below the DNA. the 5‘ and 3‘ ends of an mRNA strand produced when the DNA strand is copied. Also. designate the corresponding amino and carboxy terminal ends of a polypeptide chain translated co-linearly from the mRNA. (b) Which end of the mRNA is synthesized first? t c) Which end of the protein strand is synthesized first? A DNA l)\\ ti'aiiscrihed DNA strand l I ,4 ,, 3 fl :‘_ ,, Page Total: -l. (H) pts) (21) When the two strands in a DNA double helix in a water solution are gradually separated by slowly heating the solution. in what way does the percentage G l C of the DNA affect the temperature at which half of the DNA is single stranded and the other half is still double stranded‘.’ (b) Show a graph plotting temperature vs. absorption of UV light (260 nm) b) double stranded DNA in a water solution. as it is heated to a higher and higher temperature. allowing the DNA to gradually become increasingly single stranded. (c) Please also show a graph plotting G+C vs. Tm. _ . ~ . A 1; G tC 1g tuft“ , ’l’hfl tM \\ l9? WWW” - i’ r nu 4' i t—B ‘tenmemlure t inn 2 ii!" 3 (moo/xi _w_ _ 'TLV‘ . . 5. (3 pts) Make up any RNA sequence you care to, but show one that is 16 bases in length and is capable of folding back on itself to form a double stranded. helical RNA hairpin. 8 base pairs in length. i RTl t} \(C‘tl (THU. ’(n‘. (Is-Vi? *9 any 133A sequence that suit :old into at hairpin— “l :sll'ilCllliiLK 6. (5 pts) What single property of a primary protein sequence structure most determines how the protein will fold to become its final tertiary structure? lnc sequence ml Llama) :u 2 Page Total: ". (5 pts) What is meant when a mutation in a gene is said to be "temperature sensitive?" ‘ in product may; gene) works it: one temperature. but not at another temperature u: Lavmin-mational ehrnget ,iflt/E/«ZLU f/Le'mjiz’u" /),h {1/1“ tar/:3 (jg/cl; ft g/«m /r; woof; ' f‘ 717. 1.5 i ’ I "f ‘ ' / , I < at or Haifa/tiny ) / _. R. ( 10 pts) (3) What is the maximum number of kinds of amino acids found in most natural proteins? (b) How many different natural codon sequences are there? (c) How many of these codons don‘t code for any amino acid (i.c.. are stop codons)? '4,'.;~"\\ trml t). (5 pts) ln eukaryotesr why does alternative pre—mRNA splicing allow one gene to possibly code for more than one kind of protein? H. Winston.» ol‘i'HRN/‘t are ci'e;,ued lion: the x1: . c day :1 ft Mel/tfwiit " {75,7f'9'1m5 [ti/61d”? :1” 2'1 ,_I ‘ I V (j',/‘l‘z")t W “J\ z MZI‘L‘H BL I/l’im "MEAL/'1 {f > /":" A :l 5) 10. (5 pts) (21) For a parent eukaryotic cell dividing to become two daughter cells. draw a simple cell cycle (circular) diagram showing the time course sections of mitosis and cytokinesis in the parent cell. Label the diagram. showing when DNA synthesis occurs and when cell division occurs. (b) lfa cell has a diploid (2N) amount of DNA at the beginning of S phase, how much DNA does the cell have at the end ofthc S phase? t =1“! ceil. smréiw at {i l éSéGlémitosis-écell (lit ésion at the end M Q“ \m mt \aylliiicwlfi «mtrrs during S phase ”30‘ 4b} Diplmd (ZN l amount ol DNA doubles during r» 1'2‘,‘ u v : - A :w . , J nt‘DNr‘i ill the end (its phase. (9‘ i , - ‘ Cal / 1‘ f y, l — u r . i I (M (-71" b "622" M We? fwd 5-) (“Tum/“5‘ [JUL/2Q O a! gwt L/iij‘iA'J-i-(S 5.11 SI ”144111 i / l/L'I‘it‘é } ‘ K a ' 4 t/ ( cguddviz‘ria bub/7.: (’[.'[{' 5A."; .1 Lu!“ tit: SILL {19L [71 1/1” 75/14}: .1" "if /}; ép-[V ,’ di’iltt.6ttd ct thtaee more (0‘; J 4N ain't/wamtte t\ 3% ,4 3?, Page Total: I 1. (l0 pts) Show a time—course diagram of the different kinds of histone protein monomers as they assemble into the larger structure known as a nucleosome. l \‘m llfi-l i-3 on?H‘s—)tctrztntcr. 'l o tltis terrain-ti tin ilZ‘\—l DB dimer ix " lhen illltlliicl' MIA—l llll dimer is added in finish the making ol‘ooe nttcleomme, 7 @ HM H26 ———-———a See l inure 1-“.1). Q23}? 12. (ll) pts) (11) Name three general ways that certain amino acids. in histone protein amino terminal tails. are modified in vivo in nucleosomes in chromatin? What Specific modifications of histone tails allow other (non-histone) proteins. having either (b) a bromodomain or (e) a chromodomain. to bind to histones in nucleosomes‘.’ ~.. t N . 6M 671,1... "‘3 92 (7,5411: '.:, . M. 2m nietmlation. 11(‘Cl:~lllllt"ll. phosphor)lattioiL Lll‘lLHlllllii/lllit'll {uni} its: ot‘these iiittr neetl he nan-ti; liir lilli ermlm. the \eet) lotion ol‘spccilie lysines in ltl\lt.=11e tails, - 7% ‘PJOHTJChCl/Vla-lmg lg: \lCll1\lL!llUH ot‘spceilie iwinex in historic tails. »" "’29 Chl'anOdOl/WULK. f . l3. (5 pts} When chromatin is compacted (within certain chromosomal regions) into heteroehromatin. what al‘l'eet does this have on transcription of that region? there l\ :i retltit-tion in liatmcrimion ,L‘Ue‘lfii in the heteroelanamino region He ta mm W WC 'ien llClLlL’Ll‘ IL mkkc Mm ~5 “Canteens" Div-l ,go dianqmpmm lg Cli/ a hiqntk} l'éclkLCQOl. ...
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