ZZ electrophoresis Friday

ZZ electrophoresis Friday - Lab #3 DNA Electrophoresis Sect...

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Lab #3 DNA Electrophoresis Sect i on 1332 7 Friday 1:00pm-4:00pm ZHS 359 Zheng Zhang Office hours: T/Th 11:00am-12:00 zhengz@usc.edu
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quiz 1. please list 3 characteristics that influence the migration rate of a charged molecule in an electric field. 2. which pole does DNA run toward, positive or negative? Why r
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Assemble the Gel Assemble the Gel Box White Comb Black Dams Gel Bed
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Objective: to run electrophoresis of PCR amplified Alu insert to determine genotype for Alu insertion to perform Hardy-Weinberg analysis of class result
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Principle of Gel Electrophoresis Fig. 21.1 Watson, JD et al,. Molecular Biology of the Gene , Pearson Education, Inc. San Franscisco, 6th Ed.
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Agarose Gel Matrix
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Electrophoresis Overview Load samples in conservative wells
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Assemble the Gel Assemble the Gel Box White Comb Black Dams Gel Bed
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Loading the Gel Loading the Gel Remove the dams and combs and add Remove the dams and combs and add TAE buffer. TAE buffer. Add the loading dye to each PCR sample. Add the loading dye to each PCR sample. Take the long-tipped pipettes, and add Take the long-tipped pipettes, and add your samples to each well. your samples to each well. Lane: 1 2 3 4 5 6 7 8 Lane: 1 2 3 4 5 6 7 8 Marker +/+ +/- -/- You You Marker +/+ +/- -/- You You
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Ethidium bromide is very sensitive. toxic; maybe a mutagen. When exposed to
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ZZ electrophoresis Friday - Lab #3 DNA Electrophoresis Sect...

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