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BISC 330 Spring 2010 Lecture 3

BISC 330 Spring 2010 Lecture 3 - BISC330L:Biochemistry...

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BISC 330L:  Biochemistry Spring Semester 2010 USC Lecture:  Friday, 15 January 2010 1. Review Wednesday’s material 1. Continue Chapter 1 material…  Discovery of DNA  The Genomic revolution
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Henderson-Hasselbalch  Equation For acid AH (proton donor in neutral form): AH              A -  + H +                         K a  = [A - ][H + ]/[AH]                     logK a  = log[H + ] + log([A - ]/[AH])   - log[H+] = -logK a  + log([A - ]/[AH])                          pH = pK a  + log([A - ]/[AH])             (Henderson-Hasselbalch  Equation)                         NOTE: [A - ]/[AH] = 10  pH-pKa   [A-] = [AH](10  pH-pKa ) [AH] = [A-]/(10  pH-pKa )                             Fraction ionized (f = f A -): f  = [A ]/([A ]+[AH]) = (10  )/(1 + 10  )
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pH f i  = f A  = 10 pH-pKa /(1+10 pH-pKa ) -3+pKa 10 -3  / (1+10 -3 )   = 0.001 -2+pKa 10 -2  / (1+10 -2 )   = 0.01 -1+pKa 10 -1  / (1+10 -1 )   = 0.1 pKa 1   / (1+1)   = 0.5 1+pKa 10 / (1+10 1 )   = 0.9 2+pKa 10 / (1+10 2 )   = 0.99 3+pKa 10 / (1+10 3 )   = 0.999 Fraction ionized (f = f A -): f I  = [A - ]/([A - ]+[AH]) = (10  pH-pKa )/(1 + 10  pH- pKa )
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Graph of the data
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