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BISC 330 Spring 2010 Lecture 8

BISC 330 Spring 2010 Lecture 8 - BISC330L:Biochemistry...

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BISC 330L:  Biochemistry Spring Semester 2010 USC Lecture:  Friday, 29 January 2010 1.Finish Chapter 2 material… Predicting  α  helix and  β  stands 2. Begin Chapter 3 material… Techniques in Protein Analysis
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Predicting  α  helix and  β  strands? A, E, and L =  α  helix (not V, T, I; S, D,  and N) V, I, and T =  β  strand P disrupts both   α  helix and  β  strand G fits in both  α  helix and  β  strand -VDLLKN-
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f α  = 2.2X α  - 0.7 Count  α  promoters, N α  = Sum of  HELM AND  QRK and divide by total no. of residues (N T ), to obtain  X α  = N α /N No alpha-helix forms when there is less than 33%  alpha-promoters and very stable helix forms when  there are >75% alpha promoters.  For 50% alpha- promoters there is 40% probability of alph helix. f   α  = 2.2(.33) – 0.7 = 0 [NO = 33% or less] f α  =    2.2(.75) – 0.7 = 1 or >1 [YES = 75% or more] Example:  What is f α   when X α  is 50%  Prediction of Protein  α  Structure from Amino Acid Compositio
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  f β  = 1.5 X   β – 0.5      Count  β  promoters, N β  = Sum of  GAVIST(P)* or TAVIS G(P)* and divide by total no. of residues (N T ),  to obtain X β  = N   β /N No  β  sheet forms when there is less  than 33%  β -promoters and very stable  β
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