ProblemSet9 - 4 The following data were obtained for the...

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Chapter 12: 1. A first-order reaction has a t 1/2 of 20 min. a) What is the rate constant k? b) What time is required to form 20% of the product? c) What time is required to form 80% of the product? d) How much starting material remains after 15 min? e) Compare the rate constant for this reaction to that of the decay of 32 P, which has a half-life of 14 days. 2. For the following reaction: E P ES S E k k k + + - 2 1 1 a) What is meant by the term “enzyme-substrate complex”? b) Write a rate equation for the production of ES. c) What is the rate of product formation from ES? d) If all the enzyme is bound to substrate, what is the effect of adding more substrate on the forward rate of the reaction? 3. What is meant by (a) the steady state assumption, (b) K M , (c) k cat , (d) turnover number, (e) catalytic efficiency, and (f) diffusion-controlled limit?
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Unformatted text preview: 4. The following data were obtained for the reaction A B, catalyzed by the enzyme Aase. The reaction volume was 1mL and the stock concentration of A was 5.0mM. Seven separate reactions were examined, each containing a different amount of A. The reactions were initiated by adding 2.0uL of a 10uM solution of Aase. After 5min, the amount of B was measured. Reaction Volume of A added (uL) Amount of B present at 5min (nmoles) 1 8 26 2 10 29 3 15 39 4 20 43 5 40 56 6 60 62 7 100 71 a) Calculate the intial velocity of each reaction (in units of uM/min) b) Determine the K M and V max of Aase from a Lineweaver-Burk plot. c) Calculate k cat ....
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