Midterm2AK - MIDTERM II 114A Name KEY ID Section Number 1...

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MIDTERM II 114A Name: KEY ID: Section Number: 1. Fill in the blanks. 10X5=50 pts a. When a 60kDa and a 40kDa protein are subjected to SDS-PAGE electrophoresis, the protein with the highest mobility would be 40 kDa protein. b. Repeated cycles of the technique known as Edman degradation can reveal the complete sequence of a polypeptide containing fewer than about 100 residues. c. The amino acid glycine/proline is rarely found in an α helix. d. The term glycoprotein refers to proteins that have carbohydrate as a part of their structure. e. Oligosaccharides linked to proteins via a nitrogen atom are called N-linked oligosaccharides and are covalently bonded to the amino acid Asn . f. An example of a supersecondary structure is β barrel . g. A structurally independent unit of a protein, that forms a globular cluster and often possesses a specific function, is called a domain . h. An example of an intramolecular interaction that is important in stabilizing protein structure is H-bond/Van der Waal/hydrophobic interaction . i. An example of a disease that results from improper protein folding is Alzheimer’s disease/amyloid disease/prion disease etc. j. The subunit interface in a multisubunit protein largely consists of hydrophobic amino acids.
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2. 10 X 5=50 pts Use the following to answer 1-10 below: A) proteoglycan B) hydrophobic C) enzyme-linked immunosorbent assay D) three-dimensional shape E) N-terminal amino acid F) negative charge G) amylopectin H) cation exchange I) glyceraldehyde J) peptidoglycan K) 2-mercaptoethanol L) positive charge M) C-terminus N) anion exchange 1. One of the reasons primary structure is important for a protein is that this determines the D the molecule adopts in aqueous solution. 2. The main component of a bacterial cell wall is a J . 3. The smallest aldose is I. 4. In order for CM to act as a cation exchanger, it must have a F . 5. If antibodies to the protein being assayed are available, a(n) C can be developed. 6. Both α (1 4) and α (1 6) bonds can be found in the plant product G. 7. In order for DEAE to act as an anion exchanger, it must have a L . 8. In H chromatography, a protein mixture must be applied to the column at a low pH so that the proteins will have a net positive charge and bind to the column. 9. In SDS-PAGE electrophoresis, disulfide-linked protein subunits are separated by first reacting them with K . 10. Dansyl chloride can be used to identify the E of a protein.
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3. 4X12.5=50 pts A biochemist is attempting to separate an enzyme that is present in the crude mixture with 1000 other proteins. The size, oligomeric state, PI, enzymatic activity and ligand binding properties of this protein are listed below.
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