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AKProblemSet4 - Practice Problem Set 4 114A 2006 1.What is...

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Practice Problem Set # 4 114A; 2006 1.What is the effect of each of the following treatments on the oxygen affinity of hemoglobin A in vitro? a) Increase in pH from 7.2 to 7.4 decrease in oxygen affinity b) Increase in BPG concentration from 0.2 mM to 0.8 mM decrease in affinity c) Dissociation of a2b2 into monomer subunits decrease in affinity d) Increase in pCO2 from 10 to 100 torrs decrease in affinity 2. Some mutations in a hemoglobulin gene affect Hb A (adult hemoglobulin) and Hb F (fetal hemoglobin), whereas others affect only one of them. Why? The difference between Hb A and Hb F is the serine 143 residue. If the mutation on this residue is similar to serine, the function of Hb F will not be affected. 3. Describe the molecular feature of hemoglobin S (sickle cell hemoglobin). Explain the consequence of Hb S when oxygenated. Mutant containing valine substitution causes a linear polymerization. The pocket can not accommodate glutamic acid, and in this pocket is not present in the oxygenated form. Upon oxygenation, the hemoglobin fibers dissolve instantaneously .
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