lab+11 - the wire and pulls the wire through each of the...

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Lab 11 Bipin Bagga
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Reminder of the Semester… Nov 30 th - Dec 3 rd : Lab (Obviously cause you’re here) Dec 7 th – Dec 10 th : Practical 2 Dec 11 th : Review Session for Lecture Exam 4 Room and Time to be announced Dec 14 th : Lecture Exam 4
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Enteric Known/Unknown 1 (students work alone ) 7 known provided on TSA plates Observe, make notes on colony morphology Streak out unknown mix onto TSA plate Incubate at 37C for 24 hours Enterotube Each of the 12 compartments of an Enterotube contains a different agar-based medium. Compartments that require aerobic conditions have openings for access to air. Compartments that require anaerobic conditions have layers of paraffin wax over the media . Extending through all compartments of the enterotube is an inoculating wire. To inoculate - one simply picks up some organisms on the end of
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Unformatted text preview: the wire and pulls the wire through each of the chambers in a single rotating action. Entero-tube Make Chart Inoculate Tube Serology (students work in pairs ) Make saline suspensions of organisms from TSA plates (Salmonella & other) A (negativ e control) B (identificat ion test) C (positive control) Slid e I org. I plus saline org. I plus antiseru m antigen + antiseru m Slid e 2 org.2 plus saline org.2 plus antiseru m antjgen + antiseru m Hand-washing 2 compare total bacterial counts (NA) to enterics (EMB lac fermenters) hand washing effects? Antibiotics 2 measure zone of clearance and determine sensitivity Compare to chart Winogradsky Column examine & make wet mounts from liquid...
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lab+11 - the wire and pulls the wire through each of the...

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