Lab8 - – Dilution series and spread plating – Measure...

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Bio 207 Week 8 Lab
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Lab for today Growth Curve Water (part 2) Phage (part 2) Azotobacter (part 4) Winogradsky Column (part 4)
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Bacterial Growth Bacteria multiply by binary fission after reaching a certain size. Their maximum growth rate in a given medium at a given temerpature is constant. The time that it takes bacteria to double can be determined by constructing a growth curve.
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Growth Curve To generate a growth curve a small inoculum of bacteria is introduced into a large volume of fresh medium. Typically occurs in 3 phases. Lag Exponential or Log Stationary
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Growth Curve Construction Several methods may be used to construct a growth curve
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Unformatted text preview: – Dilution series and spread plating – Measure of turbidity • We will be using a combination of the two methods to be able to correlate turbidity with cell number. • Other factors in the medium can effect bacterial growth (i.e. antibiotics) – These factors can be bacteriostatic, meaning they stop growth but do not kill them, or bactericidal, meaning they kill bacteria. Tasks for today • Constructing growth curves (See Handout). • In between measurements – Water2 (compare samples) – Phage2 (calculate titers) – Azotobacter4 (prepare wet mount and gram stain) – Winogradsky Column4 (observe)...
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Lab8 - – Dilution series and spread plating – Measure...

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