Week_5_Lab - Did you obtain 2 isolated colonies? Was the...

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Biology 207: Week 5 Lab
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Logistics Exam 2 Friday Fall Break next week Lab will be short (< 1hr) M & T sections – during open hrs (W,Th, F 10-1 or 2-5) Today Lab 1hr Exam 2hrs
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Azotobacter
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Azotobacter Aerobic, Soil microbe Fixes nitrogen from the atmosphere Enrichment Media? To use conditions of growth to favor Azotobacter Inoculate with soil Incubate in Shaking water bath
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Winogradsky Column The Winogradsky column is a simple device for culturing a large diversity of microorganisms. Invented by Sergei Winogradsky, the device is a column of pond mud and water mixed with a carbon source and minerals. The column provides numerous gradients, from which the variety of organisms can grow. Mix Cellulose slurry, mud & minerals – pour into column – 2/3 rd full, seal with paraffin, wrap with Al foil
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Streak plate check
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Unformatted text preview: Did you obtain 2 isolated colonies? Was the plate used efficiently? Did you label the plate properly? Pepper Infusion Did you obtain isolated colonies? Was the plate used efficiently? Did you label the plate properly? Confirm colonies are all 1 type. Wet mount and Gram stain Environmental Contamination: contd (wk 3) Air-borne microbes Examine the plate visually. What changes have occurred? Record your observations. What do you conclude about the air you breathe? Environmental sample Examine the plates visually. What kinds of colonies or growth do you see? Were many types of microbes present or few? Record your observations. To be graded today Inoculate Azotobacter Winogradsky Column Show Streak Plates Good Luck for the exam....
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This document was uploaded on 01/31/2010.

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Week_5_Lab - Did you obtain 2 isolated colonies? Was the...

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