Week+7 - BIOL 207 Lab Week 7 Membrane Filter Method...

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BIOL 207 Lab Week 7 Membrane Filter Method Bacteriophages Azotobactor & Winogradsky Column
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Membrane Filter Method Rapid method of detecting coliform organisms in water Water is filtered through pores that are .45um in diameter Water is allowed to pass through, while bacteria are trapped by the filter Trapped organisms are then plated onto a media (Eosin-Methylene Blue agar)
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EMB Agar Coliform bacteria will ferment lactose turning the agar pink Exception: E. coli -> metallic green on EMB agar Eosin and Methylene Blue are selective and differential dyes that inhibit the growth of Gram-Positive bacteria Selects for Gram-Negative bacteria! Colonies can be easily counted
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Bacteriophages Viruses that infect bacteria (as discussed in lecture) Obligate intracellular parasites Must infect cells in order to survive and replicate All viruses LACK the organelles for metabolism, protein synthesis, and replication Structure of bacteriophages Learn on your own! (p. 165 in lab
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Week+7 - BIOL 207 Lab Week 7 Membrane Filter Method...

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