ch. 3 microbio - A. Basic principle [Fig. 3.14] 1....

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Barbara Moore Chapters 3/17 (partial) [Cowan-Talaro] –Tools of the Laboratory I. Overview of general laboratory techniques as applied to clinical microbiology [Fig. 3.1] A. Specimen collection [pgs. 513 – 514] B. Direct microscopic examination C. Recovering pure culture of infectious agent [Fig. 3.1] 1. Inoculation Types of culture media by function [Table 3.1; Figs.3.6 – 3.9] Animal inoculation [Insight 3.1] 2. Isolation [Figs. 3.2, 3.3] 3. Incubation 4. Inspection [Figs. 3.9, 3.10, 3.11] 5. Identification [pgs. 516 – 518] Biochemical testing Dichotomous keys [Fig. 17.6] DNA-based identification II. Overview of microscopy
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Unformatted text preview: A. Basic principle [Fig. 3.14] 1. Magnification [Fig. 3.12] 2. Resolution [Fig. 3.15] B. Light microscopes vs. Electron microscopes [Table 3.5] III. Staining specimens: Viewing microbes requires contrast [Fig. 3.25] A. Simple stains B. Differential stains 1. Gram stain: Gram positive vs. Gram negative [see also, Insight 4.2, pg. 95] 2. Acid fast stain: Diagnostic for Mycobacterium sp. IV. Study suggestions [Chapter 3] A. Case File 3 B. Multiple choice & True/False: 2 – 5, 8, 10 – 15 C. Writing to learn: 3, 10 D. Concept Mapping: 2 E. Critical Thinking: 1, 6, 7 F. Visual Understanding: 1...
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This note was uploaded on 02/01/2010 for the course BIO 226N taught by Professor Blinkova during the Spring '08 term at University of Texas at Austin.

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