microbio ch. 3- working with microbes in the lab

microbio ch. 3- working with microbes in the lab -...

Info iconThis preview shows pages 1–7. Sign up to view the full content.

View Full Document Right Arrow Icon
Chapter 3   [with materials from Chp 7, 17]  Working with Microbes in the Lab
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Relative  microbial size Metric system 1 mm = 1,000 μ m 1 μ m = 1,000 nm The need to magnify: protozoa, fungi - μ m bacteria – μ m viruses – nm
Background image of page 2
Transmission Electron Microscope
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Microscopes used in Microbiology Light microscope Maximum useful magnification ~2,000X with 0.2 μ m resolution Used to ‘see’ bacteria, fungi, protozoa Electron microscope Maximum useful magnification ~100,000 – 650,000 with 0.5 – 10 nm resolution Used to ‘see’ viruses, subcellular organelles, more detailed images of cells
Background image of page 4
Light Microscopy Like other cells, microbes are largely  water. Relevance to microscopy? Lack of contrast – can’t “see” a bacterium  on a microscope slide Solution – stain cells to enhance contrast
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Staining cells for  light microscopy Basics - ‘sticking’ cells to glass slides by heat fixing   Simple stains -  uses a single stain
Background image of page 6
Image of page 7
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 02/01/2010 for the course BIO 226N taught by Professor Blinkova during the Spring '08 term at University of Texas at Austin.

Page1 / 23

microbio ch. 3- working with microbes in the lab -...

This preview shows document pages 1 - 7. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online