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microbio ch. 3- working with microbes in the lab

microbio ch. 3- working with microbes in the lab - [,17...

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Chapter 3   [with materials from Chp 7, 17]  Working with Microbes in the Lab
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Relative  microbial size Metric system 1 mm = 1,000 μ m 1 μ m = 1,000 nm The need to magnify: protozoa, fungi - μ m bacteria – μ m viruses – nm
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Comparison of Light Microscope &  Transmission Electron Microscope
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Microscopes used in Microbiology Light microscope Maximum useful magnification ~2,000X with 0.2 μ m resolution Used to ‘see’ bacteria, fungi, protozoa Electron microscope Maximum useful magnification ~100,000 – 650,000 with 0.5 – 10 nm resolution Used to ‘see’ viruses, subcellular organelles, more detailed images of cells
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Light Microscopy Like other cells, microbes are largely  water. Relevance to microscopy? Lack of contrast – can’t “see” a bacterium  on a microscope slide Solution – stain cells to enhance contrast
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Staining cells for  light microscopy Basics - ‘sticking’ cells to glass slides by heat fixing   Simple stains -  uses a single stain -  reveals shape, size  
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