Lecture_10_Jan_27_

Lecture_10_Jan_27_ - Cytoplasm-Nucleus Transport reading...

Info iconThis preview shows pages 1–8. Sign up to view the full content.

View Full Document Right Arrow Icon
1 Cytoplasm-Nucleus Transport reading assignment: 704-712
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
2 Cytoplasm-Nucleus Transport bi-directional transport general features: (histones (in), ribosomes (out)) gated transported via a pore (nuclear pore complex posttranslational import (nuclear pore complex) on the nuclear membrane imported as folded polypeptide chains
Background image of page 2
3 Cytosol-Nucleus Transport What is the property of the nuclear membrane (i.e. nuclear envelope)? How is a cargo protein transported into or out of the nucleus? What is the machinery mediating nuclear transport?
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
4 Structure of the Nucleus The nuclear envelope is composed of two (outer and inner) membranes with the outer nuclear membrane connected to the ER membrane Nucleoplasm is the liquid equivalent of the nucleus to the “cytosol” of the cytoplasm Nucleous is a subnuclear compartment of the nucleoplasm where most of cellular rRNA is Perinuclear space is the space synthesized Nuclear lamina is a subnuclear structure near the nucleus inner membrane and it is composed of between the outer and inner membranes and it is connected to the ER lumen A nuclear pore complex is a large protein complex located at where the outer and inner nuclear membranes meet. It is the gate of the cytosol-nucleus transport. lamin (a cytoskeletal protein) and lamin-associated proteins.
Background image of page 4
5 The nucleoplasm composition is not uniform (will not be tested)
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
6 Nuclear Pore Complex (NPC) Each NPC contains ~100 different proteins (M.W. of the complex: ~125 x 10 6 kDa) nucleoporins: a major component of NPC complex (> 50 members found) lining the central pore primary mediators of the cytoplasm-nucleus transport two subtypes: one containing FG repeats and the other does not
Background image of page 6
Passive transport of small molecules through the NPC via free diffusion (recall the definition of passive transport: no energy needed) water, ions, small metabolites, small proteins (<60 kD small proteins (<60 kD) pore size estimated from the 9 nm pore size estimated from the
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 8
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 02/01/2010 for the course MCDB 103 taught by Professor Staff during the Winter '08 term at UCSB.

Page1 / 26

Lecture_10_Jan_27_ - Cytoplasm-Nucleus Transport reading...

This preview shows document pages 1 - 8. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online