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Unformatted text preview: direct effect. One way to explore this possibility would be to see whether citrate/malate exchange requires ATP, or whether it can be driven by an electrical potential difference that is set up in a different way. Further experiments have shown the latter to be the case. The last two lines of the table show that citrate leaves the matrix even more rapidly if the membrane is disrupted by a detergent. The efflux then does not require exchange for malate (line 10) and is not affected by uncouplers (line 11). 4. Detergents have a single hydrophobic chain and an ionic or polar head-group, whereas phospholipids have two hydrophobic fatty acid chains for each ionic head-group. The more bulky hydrophobic domain of phospholipids cannot be packed to form a spherical or elipsoidal micelle without exposing the fatty acid side chains to water. In the diagrams below, triangles and rectangles indicate the relative spaces occupied by the the hydrophobic parts of the molecules, relative to the polar head-groups (heavy bars). detergent micelle phospholipid...
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This note was uploaded on 02/02/2010 for the course BIOCHEMIST Biochem 44 taught by Professor Parson,young during the Winter '10 term at University of Washington.
- Winter '10