Xtra Problem Titration of Glycine

Xtra Problem Titration of Glycine - the-NH 3 form c How...

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The amino acid glycine is often used as the main ingredient of a buffer in biochemical experiments. The amino group of glycine (pK a =9.6) can exist either in the protonated form (-NH 3 + ) or as a free base (-NH 2 ) because of the reversible equilibrium: R- NH 3 + ↔ R -NH 2 + H + a) In what pH range can glycine be used as an effective buffer due to its amino group? b) In a 0.1 M solution of glycine at pH 10.2, what fraction of glycine has its amino group in
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Unformatted text preview: the -NH 3 + form? c) How much 5M HCL must be added to 1.0L of 0.1M glycine at pH 10.2 to bring its pH to 8.6? d) When 99% of the glycine is in its -NH 3 + form, what is the numerical relation between the pH of the solution and the pK a of the amino group?...
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This note was uploaded on 02/02/2010 for the course BIOCHEM Biochem440 taught by Professor Dr.klevit during the Winter '09 term at University of Washington.

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