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Unformatted text preview: e.g. if upper limit is 80kDa, all proteins with MW > 80kDa will come out together (at the beginning) lower limit : o the lower limit is determined by probability: the smaller the protein the more likely it will enter a hole (smaller > more holes > longer way). But, at a certain size the likelihood of entering a hole diverges and small Proteins cant be separated anymore e.g. if lower limit is 20kDa, all proteins with MW < 20kDa will come out together (last), because they all have to travel the longest way...
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This note was uploaded on 02/02/2010 for the course BIOCHEM Biochem440 taught by Professor Dr.klevit during the Winter '09 term at University of Washington.
- Winter '09