Hemoglobin Worksheet(solutions)

Hemoglobin Worksheet(solutions) - conformation of adjacent...

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Hemoglobin Worksheet R-State T-State 1) Match T/R states. 2) What is the difference between homotropic and heterotropic effectors? Homotropic = normal ligand and modulator are the same Heterotropic = ligand and modulator are different 3) Name 1 homotropic and at least 3 heterotropic effectors for hemoglobin. Homotropic = O 2 Heteropropic = CO 2 , H + , 2,3-BPG 4) Where do those effectors bind? Draw circles on the picture above (use either pic or both, some effector locations are easier to see in T/R state). RED = O 2 binding site, yellow = H + , green = CO 2 , pink = 2,3-BPG Note: the number of circles does not represent the number of binding sites/hemoglobin (except for 2,3 BPG which has only ONE binding site/hemoglobin, whereas O 2 , H + , and CO 2 have FOUR binding site, e.g. one per subunit) 5) Which state does each effector favor? Why?
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O 2 = binding of O 2 to one subunit causes conformational changes affecting the
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Unformatted text preview: conformation of adjacent subunits > favoring R-state CO 2 = binds at N-terminus of each subunit > favors T state by release of H + and stabilization through ionic interactions (with positively charged residue, e.g. ARG) H + = protonates HC3, favoring the T-state 2,3 BPG = binds to center of hemoglobin molecule through interactions with positively charged residues that surround pocket in T-state 6) How does each effector effect the O 2 affinity of hemoglobin? O 2 = increase CO 2 , H + , 2,3 BPG = decrease NOTE: Carbon Monoxide also acts as a modulator. Where does it bind and how does that effect the O 2 affinity of hemoglobin? Since carbon monoxide binds to the heme-group (just like oxygen) is increases the affinity for oxygen and therefore inhibits the release of oxygen in the tissues (where it can cause hypoxia)....
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This note was uploaded on 02/02/2010 for the course BIOCHEM Biochem440 taught by Professor Dr.klevit during the Winter '09 term at University of Washington.

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Hemoglobin Worksheet(solutions) - conformation of adjacent...

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