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Unformatted text preview: Effects on v max Effects on K M No inhibitor V max = k 2* [E T ] therefore only changes with the change in [E T ] Michaelis-Menten constant Intrinsic property of S-E relationship, therefore never changes Competitive inhibitor Since no effect on [E T ], no change in v max either Constant, but with competitive inhibiter we have K apparent =K M and it may seem like K M changes, but instead we observe a k 1 k 2 k-1 k-2 @ 1/2v max K M = [S] K M = apparent K M (observed K M ) new (apparent) K M (which is based on real K M ) Irreversible inhibitor Decrease in v max since inhibitor inactivates E (resembles decrease in [E T ]) NO!! K M never changes (you should know this by now ) Note: since v max is also effected the relationship @ v = 1/2v max [S]=K M still holds true...
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This note was uploaded on 02/02/2010 for the course BIOCHEM Biochem440 taught by Professor Dr.klevit during the Winter '09 term at University of Washington.
- Winter '09