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Abstract - Gr ow No Glow Biological T ransformation &...

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Unformatted text preview: Gr ow No Glow Biological T ransformation & Genetic Engineering Name: TA: Angela Strobel Lab: Tus 5:15 – 8:15 Abstract: Transformation is a biological process in which bacteria uptake plasmids from the surrounding environment that gives new characteristics to the microorganism. Such process may occur either naturally or synthetically. The objective of this laboratory experiment is to study the genetics effects of pGLO plasmid when inserted into E. coli bacteria by confirming the production of Green Fluorescent Protein (GFP) in the presence of arabinose and by confirming the resistance of E. coli to Ampicillin antibiotic. The actual results matched the predicted ones as GFP was produced only in the transformed E. coli that was incubated in arabinose. On the other hand, the transformed E. coli showed resistance to the Ampicillin antibiotic. With this basic foundation of understanding transformation and gene regulation, science has evolved better applications toward genetic engineering. Genetic engineering is important it has led to new health treatments, modified agricultural, and environmental solutions. I nt roduction: The major purpose of this laboratory experiment was to gain knowledge in transformation of genetics and its effects of pGLO plasmid when inserted into E. coli bacteria. These effects were tested in different environments. The first discovery of bacterial genetics transformation was around1930s by Dr. Fred Griffith when he was studying the pneumococcus bacteria that are the most common cause of bacterial pneumonia, associated with meningitis and other infectious diseases. Dr. Griffith has discovered that when he isolated pneumococcus bacteria from pneumonia patients, to another environment (media plate) then he noticed those bacterial colonies are growing with reasonable size. He named the original strains as smooth strains, and the transformed strain as rough strains. After that, Dr. Griffith has injected both healthy mice with those strains but only one with the smooth strain and the second mouse with the rough strain. He discovered the smooth strain cause has infected the first mouse by the lung disease, while the second has not developed any disease. So, smooth strains were virulent and causing disease, while the rough strains were aviruluent and not cause disease....
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This note was uploaded on 02/02/2010 for the course BIO 188 taught by Professor Capco during the Spring '08 term at ASU.

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Abstract - Gr ow No Glow Biological T ransformation &...

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