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practice_exam_1 - PRACTICE EXAM#1 Question 1 Part 1 What...

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1 PRACTICE EXAM #1 Question 1. Part 1. What would you expect to happen if an inhibitor of histone acetyltransferases is added to tissue culture cells (assuming it enters the cell nucleus readily)? Describe an experiment to test your hypothesis. Part 2. Using molecular cloning techniques, graduate student Steve has recently created a fusion protein linking the transcription factor Sp1 to the ligand-binding domain (LBD) of the glucocorticoid receptor (GR). Interestingly, the ability of this fusion protein to activate transcription from promoters containing the Sp1-binding sites (the GC boxes) is now completely under the control of the glucocorticoid hormone. In other words, Steve’s fusion protein stimulates transcription only when the hormone is present in the culture media. Could you provide a molecular explanation for this observation? Question 2. An electron micrograph of a mRNA:DNA hybrid duplex is shown below. Use arrows to label the genomic DNA strand, the mRNA strand and the poly(A) tail. Also, on the genomic DNA strand, label the segments that encode the introns and exons. Is the largest intron at the 5' or 3' end of the gene?
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2 Question 3. You are researching a genetic disease called Werner's syndrome and would like to analyze the
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practice_exam_1 - PRACTICE EXAM#1 Question 1 Part 1 What...

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