110.PS2.09 - 2009 MCB 110 Problem set 2. DNA analysis...

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2009 1/2 MCB 110 Problem set 2. DNA analysis methods and packaging 1. Deletion of the telomerase RNA from the mouse genome was not immediately lethal. Surprisingly, it took several generations for the mutant mice to show decreased survival. a) What is the function of the telomerase RNA? b) Why do you think several generations of the mutant mice survived? 2. Estimate the number of telomeric repeats in this T-loop structure. Assume the entire loop is comprised of repeats and the end joins the chromosome at the beginning of the repeat region. Alberts, Fig. 5-42. 3. Bacterial chromosomes have a single origin of replication but eukaryotic chromosomes have many origins. Why are there so many origins in eukaryotic chromosomes? 4. Describe an assay that was used to identify origins of DNA replication. 5. Why is a heat-stable DNA polymerase used for the polymerase chain reaction (PCR)? 6. You clone the cDNA for human albumin (1800 bp) into the
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110.PS2.09 - 2009 MCB 110 Problem set 2. DNA analysis...

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