2-25-08 - the liquid rises on the plate and takes some of...

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2/25/08 AAFS American Academy of Forensic Science Organic Chemistry Instruments Page 133 chromatography Analytical system that separates and identifies molecules William Henry Foundation of chromatography When a volatile chemical compound is dissolved in a liquid and is brought to equilibrium with air, there is a fixed ratio between the concentration of the volatile compound in air and its concentration in the liquid and this ratio remains constant for a given temperature Gas Chromatography Time a molecule takes to get through the column is the retention time Triangle is symbol for heat HPLC (High Performance Liquid Chromatography) has no gas and heat moving phase is a liquid TLC (Thin Layer Chromatography) Put a spot on a plate and put it in a glass container with liquid on the bottom then
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Unformatted text preview: the liquid rises on the plate and takes some of the spot with it Qualitative Used to identify substance by spotting a plate TLC is not a instrument it is a visual medium TLC is out of date because it isnt as accurate RF value Amount the spot moved up the plate Electrophoresis Procedure used for protein enzymes Optical method Spectrophotometry Plancks Constant E=hf E= Energy H= universal constant F= frequency Uses UV or IR absorption to detect Atomic theory When an atom gets energized by the UV or IR it kicks out an electron GC/MS (Gas Chromatography Mass Spectrometry) The GC separates the substance and then the MS sucks it in and identifies it Most accurate identifier...
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This note was uploaded on 04/03/2008 for the course CRJ 305 taught by Professor Emanuel during the Spring '08 term at Rutgers.

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2-25-08 - the liquid rises on the plate and takes some of...

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