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Unformatted text preview: the liquid rises on the plate and takes some of the spot with it Qualitative Used to identify substance by spotting a plate TLC is not a instrument it is a visual medium TLC is out of date because it isnt as accurate RF value Amount the spot moved up the plate Electrophoresis Procedure used for protein enzymes Optical method Spectrophotometry Plancks Constant E=hf E= Energy H= universal constant F= frequency Uses UV or IR absorption to detect Atomic theory When an atom gets energized by the UV or IR it kicks out an electron GC/MS (Gas Chromatography Mass Spectrometry) The GC separates the substance and then the MS sucks it in and identifies it Most accurate identifier...
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This note was uploaded on 04/03/2008 for the course CRJ 305 taught by Professor Emanuel during the Spring '08 term at Rutgers.
- Spring '08