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2-25-08 - the liquid rises on the plate and takes some of...

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2/25/08 AAFS American Academy of Forensic Science Organic Chemistry Instruments Page 133 chromatography Analytical system that separates and identifies molecules William Henry Foundation of chromatography When a volatile chemical compound is dissolved in a liquid and is brought to equilibrium with air, there is a fixed ratio between the concentration of the volatile compound in air and its concentration in the liquid and this ratio remains constant for a given temperature Gas Chromatography Time a molecule takes to get through the column is the retention time Triangle is symbol for heat HPLC (High Performance Liquid Chromatography) has no gas and heat moving phase is a liquid TLC (Thin Layer Chromatography) Put a spot on a plate and put it in a glass container with liquid on the bottom then
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Unformatted text preview: the liquid rises on the plate and takes some of the spot with it Qualitative Used to identify substance by spotting a plate TLC is not a instrument it is a visual medium TLC is out of date because it isn’t as accurate RF value • Amount the spot moved up the plate Electrophoresis Procedure used for protein enzymes Optical method Spectrophotometry Planck’s Constant • E=hf ♦ E= Energy ♦ H= universal constant ♦ F= frequency Uses UV or IR absorption to detect Atomic theory When an atom gets energized by the UV or IR it kicks out an electron GC/MS (Gas Chromatography Mass Spectrometry) The GC separates the substance and then the MS sucks it in and identifies it Most accurate identifier...
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