2-11-08 - • Vertical or reflected illumination Comparison...

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2/11/08 Microscope Optical instrument that uses a lens or combination of lenses to magnify and resolve the fine details of an object Angle of acceptance Distance from eye to object Closest an object can be that an eye can see is 25cm any closer the eye has problem focusing Image projected on retina from a microscope is a virtual image Different types of microscopes Compound microscope pg 182-183 *** KNOW THE PARTS*** Uses objective lens and ocular lens (eyepiece lens) Ex: if objective lens is 45x and eyepiece lens is 10x then total magnification is 450x Monocular One eyepiece Binocular Two eyepieces Two types of illumination Transmitted illumination- common for tissue but not solid forms
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Unformatted text preview: • Vertical or reflected illumination Comparison microscope page 184-186 Two objects magnified on one field Used mainly for ballistics Polarizing microscope Used for object of a crystalline matter Scanning Electron Microscope pg 192-195 Can go up to 100,000x Uses no lens Electron based and microwave based Stereoscopic Microscope pg186-187 Large field of view Used mainly to look at larger objects Large working distance Up to 125x not as precise as a compound micoscope Chromatic aberration No concentrated focus Spherical aberration Light coming in through edges of lens don’t focus correct...
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2-11-08 - • Vertical or reflected illumination Comparison...

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