Exp13_Prelab

Exp13_Prelab - Experiment 13: Chemical Kinetics Reaction of...

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Experiment 13: Chemical Kinetics Reaction of Crystal Violet With Sodium Hydroxide
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Learning Objectives Apply Beer’s Law Determine relationship between absorbance and concentration of crystal violet Determine the rate law and rate constant for reaction of crystal violet with sodium hydroxide Graphical Method: Integrated Rate Laws Method of Initial Rates Compare and contrast methods for determining reaction rates
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Crystal Violet (CV) Molecule that contains conjugated pi (π) bonds π bond = double bonds Conjugation= alternating single (σ) and π bonds Crystal Violet (Intense Violet Color) Colorless Product
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Colorimeter Measures the amount of light that is absorbed by a solution Amount of light absorbed is directly proportional to the concentration of absorbing molecule
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Beer’s Law Mathematical equation relating absorbance of light to concentration of molecules A = εb c A = absorbance = -log (I/I o ) ε= molar absorptivity Measure of the ability to absorb light at a particular wavelength b = path length = cuvette width (1.00 cm) c = concentration of solution
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Standard Curve: Applying Beer’s Law Prepare standards Solutions of known concentration Measure Absorbance Graph Absorbance vs. Concentration Linear regression provides mathematical relationship (Beer’s Law)
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Chemical Kinetics Field in chemistry concerned with the rates of various reactions The rate can be determined experimentally in a variety of ways, change in absorbance, pH, conductance, etc. For this experiment, we are probing the change in ABSORBANCE as CV reacts with NaOH
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Reaction Rates Definition change in concentration of a reactant or product with time
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This note was uploaded on 02/06/2010 for the course CHEM 102L taught by Professor N/a during the Spring '07 term at UNC.

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Exp13_Prelab - Experiment 13: Chemical Kinetics Reaction of...

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