ProblemSet1

ProblemSet1 - BIMM100 Fall 2009 Problem Set 1 1. You’re a...

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BIMM100 Fall 2009 Problem Set 1 1. You’re a dedicated student working on fruit flies in the lab. In order to determine its base composition, you extract the flies’ DNA and assay for the concentration of each of the bases. Unfortunately, you drop three of the tubes and only know that [A] is 12% of the total DNA. a. What percentage are the other bases (G, C, T) found in the fly genome? b. What are the ratios of purines to pyrimidines? 2. Do the following conditions cause the Tm of a given strand of DNA to increase or decrease? Explain. a. CGTTAAT Mutating the first two base pairs from CG to AT GCAATTA GC TA b. Raising the pH from 7 to 13. c. Cutting the concentration of MgCl 2 in solution by ½ . 3. True or False? Explain. a. It is possible for DNA to form secondary structures.
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b. Chargaff’s rules invalidated the tetranucleotide theory. 4. For the Kornberg experiment in which DNA polymerase was discovered, we talked about a reaction which required template DNA, dNTPs and DNA polymerase to replicate the DNA. a.
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ProblemSet1 - BIMM100 Fall 2009 Problem Set 1 1. You’re a...

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