ProblemSet4

ProblemSet4 - in vitro . b. Fill in the blank or circle the...

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BIMM100 Fall 2009 Problem Set 4 1. You want to knockout the Finger gene in yeast. a. If the gene is non-essential, how will you select for cells that contain your knockout? b. Unfortunately, it turns out the Finger gene is essential. i. What does this mean and why is it a problem for your experiment? ii. Describe another way to test for the function of the Finger gene. 2. True or False? A dominant negative mutant removes the wild type copy of that same protein from the cell. Explain. 3. Franklin, a brilliant graduate student, is studying how global gene expression in bacteria is altered in the presence of ampicillin. To do so, he is using a microarray. a. True or False? This experiment could also be performed with a northern blot or Q-PCR. Explain.
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b. Describe the chip that will be used in the experiment. c. List the steps that will be performed in the experiment. d. What result will Franklin see if all of the cell’s genes are upregulated in the presence of ampicillin? 5. RNAi a. Describe how you would make double stranded RNA
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Unformatted text preview: in vitro . b. Fill in the blank or circle the correct answer. In RNAi, (SINGLE STRANDED/DOUBLE STRANDED) RNA is cut into small pieces by the enzyme . The small pieces are called . These RNA then associate with the complex known as , where they are converted to (SINGLE STRANDED/DOUBLE STRANDED) RNA. This RNA then binds to mRNA which is degraded. c. Since the DNA is unaltered, why was RNAi taught under the category of gene inactivation? 6. You are studying human cancer cells. a. What are three characteristics of a cancer cell? b. You believe that telomerase is overactive in these cells. Explain how this can contribute to their cancerous phenotype. c. You also suspect that myc is overexpressed in these cells compared to wild type cells. To see if this is the case you choose to perform Q-PCR. i. BRIEFLY describe the steps of Q-PCR. ii. On the graph below, draw the expected results if in fact myc is overexpressed in your cancer cells....
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ProblemSet4 - in vitro . b. Fill in the blank or circle the...

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