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Unformatted text preview: in vitro . b. Fill in the blank or circle the correct answer. In RNAi, (SINGLE STRANDED/DOUBLE STRANDED) RNA is cut into small pieces by the enzyme . The small pieces are called . These RNA then associate with the complex known as , where they are converted to (SINGLE STRANDED/DOUBLE STRANDED) RNA. This RNA then binds to mRNA which is degraded. c. Since the DNA is unaltered, why was RNAi taught under the category of gene inactivation? 6. You are studying human cancer cells. a. What are three characteristics of a cancer cell? b. You believe that telomerase is overactive in these cells. Explain how this can contribute to their cancerous phenotype. c. You also suspect that myc is overexpressed in these cells compared to wild type cells. To see if this is the case you choose to perform Q-PCR. i. BRIEFLY describe the steps of Q-PCR. ii. On the graph below, draw the expected results if in fact myc is overexpressed in your cancer cells....
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- Fall '09