ProblemSet6

ProblemSet6 - BIMM100 Fall 2009 Problem 6 1. You are...

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Problem 6 1. You are studying transcription of the Problem gene and are unsure of the location of any proximal promoter elements or promoters. To identify them, you choose to search the first 500bp upstream of the Problem gene. a. Name the experiment will you perform to identify a proximal promoter region. b. This experiment involves the use of a reporter gene. (1) What are two examples of a reporter gene? (2) What is the purpose of a reporter gene? c. You make the following truncations. Here are the results from your experiment. Reporter gene Activity A: +++++ B: +++ C: +++ D: ++ No 5’ UTR: + How many proximal promoter (or promoter) regions exist in the 5’ UTR? Circle where they are found on the above diagram. d. Now that you have isolated a region of interest, you would like to see whether a transcription factor binds to that DNA, so you perform DNase I footprinting. i.
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This note was uploaded on 02/07/2010 for the course BILD 661039 taught by Professor Mel during the Fall '09 term at UCSB.

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ProblemSet6 - BIMM100 Fall 2009 Problem 6 1. You are...

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