ProblemSet7

ProblemSet7 - BIMM100 Fall 2009 Problem 7 1. Bobert, a...

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BIMM100 Fall 2009 Problem 7 1. Bobert, a graduate student in a transcription lab, has been studying the protein Bob for the past year. He knows that Bob acts as a transcription factor in vitro, but is unaware of whether it plays the same role in the cell. a. To test this, he would like to examine which DNA Bob binds to in the cell. (1) Name an experiment that will answer this question. (2) Briefly describe the steps he will take to perform this experiment. b. Describe a necessary control that Bobert will perform in this experiment. c. He finds that Bob binds to the 5’ UTR of the Trumpet gene but not to the 5’ UTR of the Clarinet gene. What will his results look like for him to make this conclusion? 2. Chromatin modification and transcription a. SIR2 is involved in histone deacetylation. (1) How does deacetylation affect chromatin structure? (2) If a cell possessed a mutant version of SIR2 that could not bind to SIR3 and SIR4, how would this affect chromatin structure?
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b. What effect would an HP1 lacking a chromodomain have on chromatin structure? c.
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ProblemSet7 - BIMM100 Fall 2009 Problem 7 1. Bobert, a...

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