{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

L7_TwoDyeGeneExp - ECEN 689 Statistical Computation in GSP...

Info icon This preview shows pages 1–6. Sign up to view the full content.

View Full Document Right Arrow Icon
ECEN 689 Statistical Computation in GSP http://www.ece.tamu.edu/~ulisses/ECEN689/ Lecture 7: Two-Dye Gene-Expression Microarrays Ulisses Braga-Neto Genomic Signal Processing Laboratory Department of Electrical and Computer Engineering Texas A&M University
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Competitive Hybridization Labeled mRNA from distinct samples are hybridized on the same slide. Two distinct channels: – Dye 1: cyanine 3 (cy3) - typical emission wavelength = 532 nm (green) – Dye 2: cyanine 5 (cy5) - typical emission wavelength = 635 nm (red) Ratios of cy3/cy5 are taken, which cancels any spot effects across the array. Recent two-dye arrays (e.g. Agilent) allow the analysis of each channel separately.
Image of page 2
Oligo Two-Dye Arrays Probes are "long oligo" (e.g. 60-base) cDNAs (as opposed to the "short oligo" 25-base probes in Affymetrix arrays). Only one long oligo probe per gene. Longer oligo probes may have free extremeties closer to the solution phase, easing the hybridization process.
Image of page 3

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Agilent Microarrays Long-oligo Arrays built by ink- jet "in situ" deposition proprietary technology.
Image of page 4
Data Files .GAL : Contains probe addressing information, control IDs and geometry information (similar to .CDF files in Affymetrix) .GPR : Expression file output by GenePix image analysis software. .SPOT : Expression file output by Spot image analysis software.
Image of page 5

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 6
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern