BSC2010L - Lab Report 2 (Genetics Actual Report)

BSC2010L - Lab Report 2 (Genetics Actual Report) - William...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
William Linkous Section 0506, Page 1 Abstract. The determination of evolution can be determined through isolating genes, using electrophoresis, and applying results to the Hardy-Weinberg Equation. In this experiment the Alu element gene, TPA -25, is isolated and tested for the amount of each allele in a lab class sample. Through using a Chi-Square test the level of adherence to the Hardy-Weinberg Equilibrium can be determined and infer whether or not evolution is occurring based on this. Introduction. One of the leading genres of study in contemporary science is evolution and its effects. Researchers have learned how to find, isolate, and analyze specific genes and alleles to determine their genotypic and phenotypic traits. With this science a plethora of knowledge about our own human genomes can hint towards evolutionary trends in an entire population. Branco et al. demonstrated this in their study of Azorean ancestry compared to Portugalian stating, “ACE and TPA-25 show the highest values of heterozygosity in both populations,” and, “…the Azores shows no significant genetic differences from that of mainland Portugal and that it is an outbred population.” (p. 223) There are many different techniques of doing this, but one particularly effective technique is something called polymerase chain reaction (PCR). This system works by amplifying (replicating) a specific DNA section of interest using DNA polymerase (in this study Taq polymerase) and a particular primer (in this study – oligonucleotide primers) which will target the DNA sequence. The DNA sequence of interest is an Alu element located on chromosome 8 called TPA-25 (tissue plasminogen activator). Alu elements are spread throughout primate DNA, and the presence of such genes as TPA-25 can
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
William Linkous Section 0506, Page 2 indicate the evolution of humans from nonhuman primates or amongst themselves. As Batzer et al. stated in their analyses of Alu insertions, “…four polymorphic Alu insertions were shown to be absent from the genomes of a number of nonhuman primates, consistent with their arising as human genetic polymorphisms sometime after the human/ African ape divergence.” (p. 12288) After analyzing the representation of alleles for TPA-25, they can be applied to the Hardy- Weinberg equilibrium in order to determine whether or not evolution has occurred. This Hardy- Weinberg Equilibrium is a keystone for determining whether or not evolution is occurring since any deviation from its standard values proves evolution. If TPA-25 is successfully isolated and analyzed for allelic frequency, I believe that the population will not break Hardy-Weinberg equilibrium. This is because our lab sample has all of the five characteristics in order to maintain
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 6

BSC2010L - Lab Report 2 (Genetics Actual Report) - William...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online