Chapter 4 - F, Y, W absorb UV (above 250 nm); also weak...

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Chapter 4 Classes: nonpolar (PALMVIPT), polar (SCATTGG), acidic (GA), basic (HAL) commonly weak, polyprotic acids (pK 1 : 2.0-2.4, pK 2 : 9.0-9.8) cysteine is most reactive AA Amino acid reactions: o amine replaces –OH of carb. group (amide) o alcohol replaces –OH of carb. group (ester: good leaving group, can form polymers) o aldehyde double bonds w/ amino group (Schiff base) o acyl chlorides can “substitute” amino group (amide) o cysteine reacts w/ self to from sulfide bridge o ninhydrin is a strong oxid. agent that is sued to detect presence of AA (purple product) D = + = clockwise, H on left; L is opposite, naturally found
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Unformatted text preview: F, Y, W absorb UV (above 250 nm); also weak fluorescence, W has phosphorescence NMR: can characterize AA; at increased magnetic fields, can be used to find 3D structure Separation and Analysis o Ion exchange chrom.: sep. based on charge (cation exchange binds cations); can remove AA by changing pH or increasing concentration of counter ion o GC: sep. based on partition eq. o HPLC: sep by forcing liquid mobile phase through column at high pressure o reverse chrom.: derivatized AA bound to stationary phase and eluted w/ increasing concentrations of a polar substance...
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Chapter 4 - F, Y, W absorb UV (above 250 nm); also weak...

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