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Unformatted text preview: • F, Y, W absorb UV (above 250 nm); also weak fluorescence, W has phosphorescence • NMR: can characterize AA; at increased magnetic fields, can be used to find 3D structure • Separation and Analysis o Ion exchange chrom.: sep. based on charge (cation exchange binds cations); can remove AA by changing pH or increasing concentration of counter ion o GC: sep. based on partition eq. o HPLC: sep by forcing liquid mobile phase through column at high pressure o reverse chrom.: derivatized AA bound to stationary phase and eluted w/ increasing concentrations of a polar substance...
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- Fall '08
- Biochemistry, Amine, amine replaces OH