Chapter 14-pt2

Chapter 14-pt2 - Chapter 14: 363 371 Translation &...

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I. Translation: Process of Protein Synthesis; mRNA translated in 5’-3’ direction & polypeptide made in N-C direction; depends on binding of each aa to its won A. mRNA Codon Recognizes the tRNA Anticodon, not the aa that the tRNA carriers 1. exp by Ehrenstein changed Cys to Ala on Cys tRNA B. Adding an Amino Acid to tRNA by aminoacyl-tRNA synthetase in aminoacylation/charging 1. produces aminoacyl-tRNA/charged tRNA 2. uses energy from ATP hydrolysis 3. process: aa + ATP aminoacyl-AMP enzyme transfers aa from C. Initiation of Translation 1. process in prokaryotes a. 2+ b. 30S binds to ribosome-binding site (RBS aka Shine-Dalgarno seq) on mRNA i. purine-rich AGGAG (or similar) RBS complementary to pyramidine-rich UCCUCC (always) at 3’ end of 16S rRNA ii. usu 8-12 nucleotides upstream of AUG initiation codon iii. exp done w/ compensating mutations to prove c. initiator tRNA binds to AUG start codon/P site IF3 is released i. prokaryotes have fMet (formulated by transformylase) ii. tRNA.fMet coded by a different gene than tRNA.Met d. 50S large subunit binds
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Chapter 14-pt2 - Chapter 14: 363 371 Translation &...

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