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Oceanoptics - Ocean Optics for CH142 2010 Ocean Optics Spectrophotometers with Vernier Data Acquisition Software The word spectrophotometer and

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Ocean Optics for CH142, 2010 page 1 Ocean Optics Spectrophotometers with Vernier Data Acquisition Software The word spectrophotometer and spectrometer are used interchangeably in this handout. The absorbance of a sample is given as A = log I o I , where I o is the intensity without sample present and I is the intensity with the sample in the light path of the spectrophotometer. This calculation is done at each wavelength in the spectrum. The value of I o depends on wavelength and must be determined by placing a reference or blank sample in the instrument. The terms reference ” and “ blank ” are used interchangeably. The reference is usually a cuvette filled with only the solvent or the buffer for the experiment. The process of acquiring the reference spectrum is called calibration in Logger Pro. Every time you switch solvents or buffers, a new reference spectrum must be taken, so the instrument must be recalibrated. 1. Plug the wire coming from the power cord into the spectrophotometer to start warming up the device. Log into your computer. Select the Logger Pro icon at the bottom of the screen. 2. The spectrophotometer should be automatically recognized by the software showing the colors of the visible spectrum in the data window with Wavelength as the horizontal axis. If the visible spectrum is not displayed check your connections. If it is not a connection issue go to “Experiment” (top menu), select “Connect Interface” Spectrometer” Scan for Spectrometers”. If you wish to disable the visible rainbow spectrum you click once on the graph then on “Options” (top menu), slide down to “Graph Options.” Unselect “Draw visible spectrum.” 3. Click on “Experiment” (top menu), slide the mouse down to “Set Up Sensors”, and go across to Spectrometer:1”. The Spectrometer dialog box will be displayed. Enter a zero for
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This note was uploaded on 02/09/2010 for the course CH CH242 taught by Professor Katz during the Spring '10 term at Colby.

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Oceanoptics - Ocean Optics for CH142 2010 Ocean Optics Spectrophotometers with Vernier Data Acquisition Software The word spectrophotometer and

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