DNA - BC 367 Biochemistry of the Cell I DNA on Trial...

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BC 367 Biochemistry of the Cell I DNA on Trial November 24, 2009
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Forensic Bloodstain Analysis Is it blood? Whose is it?
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Re a c tio n with  luminol HC HC C H C C C NH 2 C N N C O H H O Is  it b lo o d? C e rta in c he m ic a ls  re a c t with b lo o d fa irly  s pe c ific a lly.
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Re a c tio n with  luminol HC HC C H C C C NH 2 C N N C O H H O Is  it b lo o d? C e rta in c he m ic a ls  re a c t with b lo o d fa irly  s pe c ific a lly.
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80%  o f po pula tio n a re  “s e c re to rs .” ABO  typing Who s e  b lo o d is  it?         43%          42%        12%        3%
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DNA Pro filing Who s e  is  it?
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Re s tric tio n Fra g m e nt Le ng th  Po lym o rphis m  Ana lys is O R Mito c ho ndria l DNA S e q ue nc ing O R Po lym e ra s e  C ha in Re a c tio n  via  S ho rt Ta nde m  Re pe a ts
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Target Region c hro m o s o m e c e ll nuc le us Do uble  s tra nde d  DNA m o le c ule Individua l  b as e s DNA in the  C e ll
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A G C T The   o rde r  o f  the   b a s e s   (A,  G ,  C ,  a nd  T)  is  uniq ue  to  a  pe rs o n.
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De te c ts  diffe re nc e s  in  non-coding   DNA tha t c o nta ins  s ho rt re pe a te d  s e q ue nc e s  ( tandem repeats ) Ta rg e t DNA is  c ut b y re s tric tio n  e nzym e s , le a ding  to  pie c e s  tha t va ry in  s ize  a m o ng  individua ls  ( R e s tric tio n  F ra g m e nt  L e ng th  P o lym o rphis m ) Nuc le a r DNA Ana lys is   RFLP Ana lys is
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R estriction F ragment L ength P olymorphism
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Nuc le a r DNA Ana lys is PC R-S TR  Ana lys is S im ila r  to   RFLP  b ut  no   c utting ; amplification  ins te a d.
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DNA - BC 367 Biochemistry of the Cell I DNA on Trial...

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