9-16-09_lecture_proteinID3A

9-16-09_lecture_proteinID3A - If someone points out to you...

Info iconThis preview shows pages 1–7. Sign up to view the full content.

View Full Document Right Arrow Icon
If someone points out to you that your pet theory of the universe is in disagreement with Maxwell's equations - then so much worse for Maxwell's equations. If it is found to be contradicted by observation -well, these experimentalists do bungle things sometimes. But if your theory is found to be against the second law of thermodynamics I can give you no hope; there is nothing for it but to collapse in deepest humiliation. -- Arthur S. Eddington (British Astrophysicist, 1882-1933) In this house, we obey the laws of thermodynamics! -- Homer Simpson
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Announcements First test this Friday, Sep. 18, 7:30 am in Lilly 1105 (this room). Exam will cover through today’s lecture Please see me or the TAs if you have questions about the material
Background image of page 2
What are the steps in protein folding and self-assembly? Scenario 1: Sequential folding. Scenario 2: Random compaction then formation of secondary structure. Actual folding is between these two extremes, simultaneous local formation of helices and sheets through local hydrogen bonding and hydrophobic interactions.
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Larger proteins and more evolved proteins often consist of domains (modularity) PLC is involved in many intracellular signaling pathways triggered by activation of membrane receptors. Mammalian phospholipase C (PLC) consists of 4 domains: Catalytic domain from bacterial PLC Synaptotagmin (Ca2+ sensor) Recoverin (Ca2+ triggered receptor) Troponin C (binds Ca2+ to produce movement in muscle)
Background image of page 4
Chromatography can isolate proteins by specific characteristics: ion-exchange, gel-filtration, affinity Ion-exchange: Use charged matrix to attract oppositely charged proteins. Can vary pH of solvent to vary protein charge (Why?) Gel-filtration: Use different pores to filter by size Affinity chromatography: Used to catch proteins that interact with a specific target. Attach the target (insulin, dopamine, etc) to the matrix. Interacting proteins (and potentially protein complexes) will be caught.
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Gel electrophoresis Can be one or two dimensional Separate proteins by size and/or isoelectric point
Background image of page 6
Image of page 7
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 25

9-16-09_lecture_proteinID3A - If someone points out to you...

This preview shows document pages 1 - 7. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online