11-16-09_Operon_Control_Gene_ExpressionB

11-16-09_Operon_Control_Gene_ExpressionB - New from...

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New from Madonna! Personal Genome Kits Tired of borderline microarray results? Here’s your lucky star!
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Announcements Quiz 8 is available. It is due Wednesday 11/18 at noon. It covers DNA structure, replication, and repair. Exam 3 is this Friday, 11/20. It will cover through translation (midway through today’s lecture). Answers to the review questions will be provided Monday.
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Summary of initiation in prokaryotes or eukaryotes Small ribosome subunit binds to mRNA bound in a complex Initiation factors bind to small subunit One factor is bound with GTP (IF2-GTP in prok. or eIF2-GTP in euk.) rRNA recognizes AUG of mRNA 5’ → 3’ tRNA-Met binds IF2-GTP and small subunit tRNA-Met enters P site GTP hydrolyzed, IF2-GDP (or eIF2-GDP) released, and large subunit binds.
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Summary of elongation EF-GTP and aa-tRNA form a complex aa-tRNA binds to codon in A site and has position adjusted using energy from GTP Peptide bond forms with peptide in P site using peptidyl transferase. Peptide released from P site tRNA Translocation of mRNA one codon, using GTP Translocation of tRNA and peptide in large subunit. From P to E site and A to P site. “Uncharged” tRNA in E site exits New aa-tRNA enters A site to re-start cycle
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Summary of translation 1) Small subunit binds mRNA 2) rRNA recognizes AUG on mRNA 1) tRNA-Met enters P site and binds to AUG 1) Large subunit binds (GTP) 2) Second tRNA binds A site (GTP) 3) Peptide bond (peptide attached to 2nd tRNA) 4) Translocation by 1 codon towards 3’ end (GTP) 5) Release of 1st tRNA from E site 6) Continue 5-8 until stop codon is reached 7) Stop codon in A site -> termination (GTP)
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Where? When? How much? Control of gene expression The power to destroy a planet is insignificant compared to the power to control mitichlorian expression. Darth Vader, re-edit
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This note was uploaded on 02/09/2010 for the course BIOL 230 taught by Professor Bartlett,e during the Fall '08 term at Purdue University-West Lafayette.

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11-16-09_Operon_Control_Gene_ExpressionB - New from...

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