Bio 100 Lab - Biochemical Reactions- Enzymes Lab Report...

Info iconThis preview shows pages 1–4. Sign up to view the full content.

View Full Document Right Arrow Icon
Biochemical Reactions- Enzymes Lab Report Part I: Graphs and Data Time Course: Absorbance vs. Time (Experimental and Control Data) Time (minutes) Experimental ABS @ 405nm Control ABS @ 405nm Exp. ABS Minus Control ABS Micromoles p-Nitrophenol 0 0.062 0.026 0.036 0.0020 10 0.186 0.025 0.161 0.0075 20 0.341 0.032 0.309 0.0175 30 0.488 0.026 0.462 0.0185 60 0.925 0.030 0.895 0.0405 Standard Curve Micromoles p-Nitrophenol Absorbance @ 405nm 0.0000 0.000 0.0025 0.038 0.0050 0.064 0.0100 0.155 0.0200 0.499 0.0400 0.852 Temperature Data Temperature (*C) Experimental ABS @ 405nm Control ABS @ 405nm Exp. ABS Minus Control ABS Micromoles product/15 min 0 0.156 0.022 0.134 0.00625 24 0.385 0.024 0.361 0.01625 37 0.643 0.031 0.612 0.02750 80 0.618 0.558 0.060 0.00050 pH pH Experimental Control ABS Exp. ABS Micromoles
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
ABS @ 405nm @ 405nm minus Control ABS product/15 min 3 0.227 0.045 0.182 0.00850 4 0.494 0.051 0.443 0.02500 5 0.660 0.044 0.616 0.02750 6 0.450 0.038 0.412 0.00185 7 0.166 0.037 0.129 0.00550 Substrate Concentration [Substrate] (mMolar) Experimental ABS @ 405nm Control ABS @405 nm Exp. ABS minus Control ABS Micromoles product/15 min 0.10 0.178 0.011 0.167 0.0072 0.50 0.370 0.014 0.356 0.0160 2.00 0.562 0.034 0.528 0.0238 4.00 0.621 0.049 0.572 0.0248 6.00 0.662 0.067 0.595 0.0265 Substrate concentration plus inhibitor [Substrate] (mMolar) Experimental ABS @ 405nm Control ABS @405 nm Exp. ABS minus Control ABS Micromoles product/15 min 0.10 0.038 0.020 0.018 0.0008 0.50 0.127 0.034 0.093 0.0092 2.00 0.232 0.052 0.180 0.0085 4.00 0.255 0.063 0.192 0.0089 6.00 0.411 0.070 0.341 0.0155 Part II: Introduction and Questions 1a) Please provide a general introduction to enzymes and their function.
Background image of page 2
An enzyme acts as a catalyst, a chemical agent that speeds up a reaction without being used up by the reaction. Without enzymes, metabolic pathways would be hindered because many reactions would take a long time. In every chemical reaction, it involved bond breaking and bond forming between molecules. To reach the transition state where bonds can change, reactants have to absorb energy from their surroundings. When the product forms, energy is released as heat, and the molecules return to stable shape. Organisms use catalysis because high temperatures would denature proteins and kill cells. Also, energy in the form of heat would speed up all reactions. By lowering the activation energy, an enzyme catalyzes a reaction by enabling the reactants to absorb enough energy to reach the transition state without the need of high temperatures. Enzymes cannot change the free energy in a reaction, but enzymes can make reactions faster in reactions that would occur eventually. Enzymes are also extremely specific for the reactions they participate in. The enzyme-substrate complex occurs when an enzyme binds to its substrate in
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 4
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 02/10/2010 for the course BIO 100 taught by Professor Shannon during the Spring '10 term at CUNY Brooklyn.

Page1 / 11

Bio 100 Lab - Biochemical Reactions- Enzymes Lab Report...

This preview shows document pages 1 - 4. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online