Practice 2_09 - 1) Describe the key steps in isolation of...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
1) Describe the key steps in isolation of cell division cycle mutants in yeast. 2. What are the consequences of a single crossover in the inverted region of a heterzygous cross for a) a pericentric inversion b) a paracentric inversion ? Give your answer in terms of acentrics, dicentrics, duplications and deficiencies and inverted sequences 3. What does it mean to say each strand of DNA has polarity? 4. (4 pts.) An auxotrophic mutant of E. coli cannot synthesize the amino acids alanine and methionine ( ala-, met- ). What do you need to add to the minimal media in order for this strain to grow? 5. Consider recombination. What are the possible ways in which the recombination junction can be resolved. Include a drawing with flanking markers to distinguish the products. 6. The two chains composing a DNA double helix unwind, and a replication fork is formed. Chain A is presented to the DNA polymerase in the direction 3' 5'. Its complementary chain, chain B, is presented in the direction 5' 3'. Answer the following. A
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 4

Practice 2_09 - 1) Describe the key steps in isolation of...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online