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Unformatted text preview: Andrew T. Hedrick Chem 32 Section S 11/8/02 Experiment 9 Separation Techniques: Chromatography Purpose: Understand the principles of chromatographic techniques in the separation of components in a mixture. Apply horizontal paper chromatography to the separation of the components in food colorings. Apply ascending paper chromatography to the separation of cations in a solution. Apply ascending paper chromatography to the separation of amino acids in a solution. Detect colorless components by reaction to produce colored product. Identify components of a mixture by comparison of retention values (R f ) and colors of the components with those of known standards. Experimental Plan: The instructions for this plan were based on The Thompson General Chemistry 2 Lab Manual, pages 101-111. Changes included omitting Part II of the experiment and using a hot plate to expedite the drying process in...
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