Lab 10[1] - Crystallization and Examination of a Protein Jonathan Sosa and Judy Garcia 1 Crystallization and Examination of a Protein University of

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Crystallization and Examination of a Protein Jonathan Sosa* and Judy Garcia 1
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Crystallization and Examination of a Protein University of Texas at Austin Department of Chemistry and Biochemistry Introduction Crystallization is a technique used to provide large crystals that can be studied and processed with X-ray crystallography. The main objective of our experiment is to obtain crystals of lysozyme using X-ray crystallography. They method we are using is called sitting drop to create the crystals. Other methods are hanging drop, sandwich drop, or free interface methods. The crystals should be different sizes based on the concentration of the drops. Water should evaporate from the protein solution, therefore, causing the concentration of the protein solution to increase, allowing crystals to form. Once the crystals are formed on the microbridges, they will be observed using a light microscope. Izit dye will be used after the first observation of the crystals. The purpose of the staining is because it will only stain the crystals that are proteins blue, the salt
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This note was uploaded on 02/14/2010 for the course CH 369T taught by Professor Mcdonald during the Spring '09 term at University of Texas at Austin.

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Lab 10[1] - Crystallization and Examination of a Protein Jonathan Sosa and Judy Garcia 1 Crystallization and Examination of a Protein University of

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