ChromatographySeparation of biomolecules is based on theirphysicochemical characteristicspolarity (solubility, volatility, adsorption) HIC, RPsize/mass (diffusion, sedimentation)GFionic characteristics (charge) IEXshape (ligand binding, affinity)Affinity chrbased on these properties the moleculesdifferentially separate between the stationaryphase and mobile phase
Liquid chromatography (LC)IEX anion exchangecation exchangechromatofocussingAffinity chromatographygroup separations vs. specific interactionsHIC (hydrophobic interaction chr.) /RPGel filtration/size exclusion; noninteracting & medium resolutionadsorption chromatogr.&highresolution
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