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Chromatography - Protein chromatography Ion exchange...

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Protein chromatography 9 Ion exchange chromatography 9 Affinity chromatography Jaana Vesterinen Institute of Biomedicine, University of Helsinki, 2008
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Chromatography Separation of biomolecules is based on their physicochemical characteristics polarity (solubility, volatility, adsorption) HIC, RP size/mass (diffusion, sedimentation) GF ionic characteristics (charge) IEX shape (ligand binding, affinity) Affinity chr based on these properties the molecules differentially separate between the stationary phase and mobile phase
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Liquid chromatography (LC) IEX anion exchange cation exchange chromatofocussing Affinity chromatography group separations vs. specific interactions HIC (hydrophobic interaction chr.) /RP Gel filtration/size exclusion; noninteracting & medium resolution adsorption chromatogr. & high resolution
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