lecture19 - Electrophoresis and Capillary Electrophoresis...

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Electrophoresis and Capillary Electrophoresis Homework: 1, 2, 3 Chapter 30
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Electrophoresis 1. Capillary Electrophoresis 2. Gel Electrophoresis in Bio-applications Science, Nov. 12, 2004
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Modes of separation capillary electrophoresis 1. Capillary Zone electrophoresis 2.Capillary iso-electric focusing 3. Micellar electrokinetic Capillary chromatography
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Capillary Electrophoresis 1. Introduction a. electrophoresis: the migration of ions in solution under the influence of an electric flied. - - - - - + + + + + + f u ep q E qE = f u ep Accelerating force Frictional force q : charge E : electric field strength f: friction coefficient u ep : velocity of the ion of electrophoresis migration b. Electrophoretic mobility u ep = E q f μ ep E Electrophoretic mobility ep = q f E = u ep
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Electrophoretic mobility ( μ ep ) is a constant and a intrinsic property of an ion. It is dependant on the charge and 3-D structure of the ion. i. Molecules of similar size, the magnitude of the mobility ( ep ) increases with charge ii. For a spherical particle of radius r moving through a fluid of viscosity η , the friction coefficient f is Stokes equation: f = 6 π η r ep = q 6 r
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C. Capillary electrophoresis
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This note was uploaded on 02/16/2010 for the course CHEM 589 taught by Professor -1 during the Fall '02 term at Penn State.

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lecture19 - Electrophoresis and Capillary Electrophoresis...

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