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DernburgLecture4_2-5-10_forprinting - Last time...

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Introduction to vesicle-mediated protein trafficking Synthesis of proteins by ribosomes attached to the Endoplasmic Reticulum (ER) Translocation into the lumen of the ER Glycosylation within the ER and Golgi Anterograde transport from the ER to the Golgi Transport from the Golgi to the cell surface (plasma membrane) or other organelles Last time: Cisternal maturation within the Golgi Receptor-mediated endocytosis Vesicle fusion mechanisms Vesicle budding mechanisms Today: 1 Coat proteins and their interactions with receptors that bind to cargoes specify the contents of specific vesicles 2 SNARE proteins mediate recognition and fusion between transport vesicles and their target membranes. A SNARE on the vesicle is called a v-SNARE, while t-SNAREs are on the target . General principles of vesicle fusion v-SNAREs and t-SNAREs form very tight coiled-coil protein complexes that facilitate membrane fusion. 3 NSF (N-ethylmaleimide Sensitive Factor) is an ATPase required for all membrane fusion events. The NSF gene in Drosophila is called “ comatose ” because all neuronal signaling stops at elevated temperature 4 Summary of anterograde and retrograde transport between the ER and cis -Golgi network 5 Mis-sorted proteins can be retrieved to their proper compartments through retrograde transport 6
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Vesicles within the Golgi network are mostly RETROGRADE, rather than ANTEROGRADE, as once believed 7 Protein trafficking through the Golgi, and cisternal maturation http://www.youtube.com/watch?v=rvfvRgk0MfA 8 Maturation of Golgi cisternae requires retrograde transport to
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