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Dernburg_Lecture3_R_Q - Reading for Protein...

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Reading for “Protein Trafficking” (Dernburg Lecture 3, February 3, 2010) Please read Lodish, Chapter 14. You can skip section 14.6 at the end if you like (I will probably not cover this in the lecture). Pay special attention to Figure 14-4 (p. 584) since I will focus on the genetic analysis of secretion in yeast as an example of how genetics is used to investigate cell biological processes. You may also find it helpful to read the historical perspective describing the analysis of the secretory system through yeast genetics, written by Randy Schekman in honor of his receipt of the Lasker Award in 2002. I’ve posted this in the folder called “Supplemental (not required) reading.” If you want to read the original paper that presented epistasis analysis of yeast secretory mutants, I have also posted it (Kaiser_Schekman_1990). Study/review questions: 1. The discovery of green fluorescent protein (GFP) has greatly facilitated living cell experiments because a. GFP is green.
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