2ALecture 14

2alecture 14

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Unformatted text preview: 2/9/10 Translation DNA RNA Protein Lecture 14 More on translation 1. Overview of Translation 2. The Translation Machinery 3. The Mechanics of Translation A-T-G-T T-G-C-A DNA 5’ A-U-G-U RNA 3’ AA1-AA2-- PROTEIN Gene to protein Gene Transcription 5' A UGA C A U U C A G U A C U CUAA C A Translation NH3+ 3' DNA polymerase moves from the 3’ to the 5’ end of its template strand. RNA polymerase moves fro the 3’ to the 5’ end of its template strand. How does the ribosome move? From the 3’ to the 5’ end of the mRNA From the 5’ to the 3’ end of the mRNA Protein COO- Overview of translation aminoacyl tRNAs* ribosome mRNA mRNA initiation elongation *tRNA part not shown Translation machinery •  tRNAs tRNA structure carriers of amino acids amino acid here to make “aminoacyl tRNA” 3’OH cartoon of a tRNA termination anticodon Guides amino acid to the right codon 1 2/9/10 •  Aminoacyl tRNA Synthetases Enzymes that join amino acids to tRNAs. Each recognizes an R group and an anticodon. NH3+ H O C C OH CH 3 What’s “aminoacyl” ? NH3+ H O C C O CH 3 NH3+ H O C C O CH 3 ΔG ~ 0 H O NH3+ C C O OH CH 3 -ΔG H O NH3+ C C O CH 3 ATP H2O P~P -ΔG 2Pi AMP ~A ~A P M U GC U GC Aminoacyl-AMP P M U GC a. a. activation charging of tRNA Aminoacyl-tRNA 1 synthetase --> 2 reactions...
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