Lecture7CM Fall09FINAL - Just as DNA Ligase links together...

Info iconThis preview shows pages 1–11. Sign up to view the full content.

View Full Document Right Arrow Icon
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Group and One-on-one tutoring Check out our website at https://netfiles.uiuc.edu/ro/www/Ligase / Just as DNA Ligase links together the gaps in the DNA backbone, our organization links together students who share an interest in Molecular and Cellular Biology and provide a medium for students to pursue and discover the plethora of fields associated with MCB. LIGASE group tutors will meet twice each week in 420 NHB at the following times: Tuesday: 5:00-6:00 PM Thursday: 4:00-5:00 PM
Background image of page 2
Reminder: EXAM 1 is one week from today. The exam will cover material presented up to and including Friday, Sept. 11.
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
MCB 250 Fall 2009 Lecture 7 Protein Folding and Analysis
Background image of page 4
The Folding Problem How do you get from this: Hemoglobin β -subunit:1 mvhltpeeks avtalwgkvn vdevggealg rllvvypwtq rffesfgdls tpdavmgnpk vkahgkkvlg afsdglahld nlkgtfatls elhcdklhvd penfrllgnv lvcvlahhfg keftppvqaa yqkvvagvan alahkyh 147 α -subunit:1 mvlspadktn vkaawgkvga hageygaeal ermflsfptt ktyfphfdls hgsaqvkghg kkvadaltna vahvddmpna lsalsdlhah klrvdpvnfk llshcllvtl aahlpaeftp avhasldkfl asvstvltsk yr 142 To this:
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
The Folding Problem How do you get from this: Hemoglobin β -subunit:1 mvhltpeeks avtalwgkvn vdevggealg rllvvypwtq rffesfgdls tpdavmgnpk vkahgkkvlg afsdglahld nlkgtfatls elhcdklhvd penfrllgnv lvcvlahhfg keftppvqaa yqkvvagvan alahkyh 147 α -subunit:1 mvlspadktn vkaawgkvga hageygaeal ermflsfptt ktyfphfdls hgsaqvkghg kkvadaltna vahvddmpna lsalsdlhah klrvdpvnfk llshcllvtl aahlpaeftp avhasldkfl asvstvltsk yr 142 To this:
Background image of page 6
Protein Folding All of the information for proper folding is present in the amino acid sequence Given the free rotation around the α -carbon, proteins can assume an essentially infinite number of conformations Some conformations are much more energetically favorable than others Usually only one conformation, the one with the lowest free energy, is the active or native state
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
How Does a Protein Find the Energetically Favorable State Does it randomly sample different conformations? Assume each residue can have three possible conformations (very low estimate) Therefore a 260 amino acid protein has 10 124 possible conformations Assume 10 -13 seconds to transition from 1 state to another Therefore it would take ~10 100 years Age of the universe = ~10 10 years
Background image of page 8
General Concepts in Protein Folding Folding of localized secondary structure is rapid Random coil to α -helix takes <10 -6 sec Folding is cooperative Tertiary structure can stabilize secondary structure Zip and Assemble (ZA) – local structures can form quickly and then assemble into global structures Domains fold independently Protein folding can take place as the protein is being synthesized N-terminal regions fold before (in the absence of) the C terminal regions
Background image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
General Concepts in Protein Folding For many proteins there are probably many pathways for folding – most molecules end
Background image of page 10
Image of page 11
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 02/17/2010 for the course MCB MCB 250 taught by Professor Miller during the Fall '09 term at University of Illinois at Urbana–Champaign.

Page1 / 40

Lecture7CM Fall09FINAL - Just as DNA Ligase links together...

This preview shows document pages 1 - 11. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online