4 - TA: Christine Feng hufeng@ucsd.edu Section: Mon 4-5...

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TA: Christine Feng hufeng@ucsd.edu Section: Mon 4-5 Center 205 OH: Wed 11-12 Geisel S&E This Week in Lecture: Anfinsen Experiment Molten Globule & Protein Folding Funnel Prions GroEL/ES Protein Stability Ch 6, 8 Anfinsen Experiment Tested ribonuclease to see if protein folding was dictated by the primary sequence Reagents: o Urea – interact with water and other molecules to disrupt hydrophobic interactions o β -mercaptoethanol – reducing agent to break disulfide bonds Separate mixture using dialysis tube (porous) which effectively separated the smaller molecules from the larger protein Found that primary sequence was enough for small globular proteins to fold into native state Free Energy Funnel for Protein Folding Entropy – drives towards unfolded state with hydrophobic residues exposed Enthalpy – drives toward folded state with hydrophobic interactions, H bonding, ionic interactions Free energy is the difference – drives towards folded state
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This note was uploaded on 02/17/2010 for the course BIBC BIBC 100 taught by Professor Buehler during the Spring '09 term at UCSD.

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4 - TA: Christine Feng hufeng@ucsd.edu Section: Mon 4-5...

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