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bioeMCB C142-fall06-mt2-Rokhsar-soln

bioeMCB C142-fall06-mt2-Rokhsar-soln - Name ID GSI Name MCB...

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Name _________________________________________ ID ______________________ GSI Name ------------------------------------------ 1 MCB 142 second midterm: Molecular Genetics Please write your name, ID, and TA’s name on the top of ALL 8 pages of this exam. Please put all your answers in the spaces below. Please use pen to write your answers. If you use pencil, you cannot request a re-grade. You may not use any calculator, cellular phone, internet connection, playstation, or other electronic device during this exam. Please double check that you have written your name, ID, and TA’s name on top of every page in the exam. Exam format: There are ten short answers (4-7 points each, total of 45 points), and five problems (10-15 points each, total of 60 points). The whole exam is worth 100 points. You have 90 minutes to complete this exam. Please pace yourself, and don’t get bogged down in any one problem (especially the short answers, where typically you will either know the right answer, or you won’t). Good luck. For Grader’s Use Only Page 2 score (out of 24) ______ Page 3 score (out of 21) ______ Page 4 score (out of 15) ______ Page 5 score (out of 10) ______ Page 6 score (out of 10) ______ Page 7 score (out of 10) ______ Page 8 score (out of 10) ______ TOTAL SCORE (out of 100) _______
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Name _________________________________________ ID ______________________ GSI Name ------------------------------------------ 2 1. Replication (4 points). DNA replication is “semiconservative.” This means (a) one strand watches Fox News, the other CNN (b) in a given double helix, one strand is inherited directly from the parental helix and the other strand is newly synthesized as its complement (c) both strands are inherited from the parent, but DNA repair removes half of the sequence randomly, keeping (“conserving”) the other half. (d) If a (previously unlabeled) bacteria is grown in the presence of labeled nucleotide precursors, half of all future descendents will have labeled DNA ANSWER: B. 2. Human genome (4 points). True or False: “Exons make up only a small fraction (<5%) of the human genome.” ANSWER: TRUE 3. Restriction enzymes (4 points). Restriction enzyme recognition sites are often palindromic because (a) DNA is an antiparallel double helix, so the two strands are the equivalent (b) In a recognition site the number of A’s is the same as the number of T’s, and the number of G’s is the same as the number of C’s. (c) Restriction enzymes typically bind to DNA as dimers (d) Processing an mRNA requires the spliceosome, which “restricts” the genomic contribution to a gene by removing introns ANSWER: C 4. Start codon (4 points) . True or False: “Every mRNA begins with the “start codon” AUG.” ANSWER: FALSE. Only the coding sequence starts with AUG – there is typically additional 5’ untranslated (UTR) sequence 5. Translation (4 points). Which of the following element(s) are essential for the process of translation from a mature mRNA? For full credit, be sure to identify ALL elements.
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